Place it at the end of a scare sequence, after a Phasmophobia ghost attack and just before the reveal that nothing was there, so the reply closes the scene and sets up a follow-up clip.
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This short clip lasts just over two and a half seconds and delivers the line 'I ain't afraid of no ghosts' in a confident, playful voice. It feels bold and cheeky, like a dare answered with a grin rather than a shiver, and it works as a quick burst of bravado whenever something spooky, strange or supposedly haunted comes up.