Place it at the start of a confused-character moment, then follow it with the No No Wait Wait sound when the reply turns into a panic, or with the Oh My God Wow sound when the other person finally notices them.
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This is a sub-second voice clip of a small, plaintive voice asking 'Are you my mommy?' in a direct, childlike way. It lands as funny and a little awkward, because the needy question sounds out of place in an adult conversation, and the sudden innocence makes people smile before they answer.