This clip is mainly used online as a quick reaction insert rather than for a clearly documented source. Short 'bro' sounds have circulated across meme edits, group chats, short-form videos and gaming compilations for years because the word is instantly recognizable and easy to fit into almost any setup. Its staying power comes from how flexible it is: creators use it for disappointment, confusion, sarcasm or mock disbelief, often in the same kind of reaction-heavy edits that also use awkward cricket or chipmunk laugh.