Place it at the peak of a scare or reveal, after Dun Dun Dun has built the tension and just before the visual payoff lands. Follow it with Vine Boom to punctuate the aftermath and reset the scene for the next gag.
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This clip is a woman's deranged scream lasting just under five seconds, raw and shrill with a frantic, unhinged edge that rises and breaks as it goes on. It feels alarming, chaotic and darkly funny, the kind of sound that makes a calm moment snap into panic in an instant.