Place it right after the damage moment in a fail compilation, then follow it with a Roblox Oof for a second reaction, and finish with a No God Please No for the full defeat sequence.
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This half-second clip is a single hurt voice line from Divinity: Original Sin 2, a brief pained 'Ay' delivered as a character takes damage. It feels sudden, silly and oddly dramatic for such a tiny sound, turning a small hit into a comic moment. The line comes from the game's hurt voiceovers, one of its more random reactions to being struck in combat.