Place it at the exact frame of the goofy reveal in your edit, after the setup shot and before the on-screen reaction. Pair it with a cartoon boing or a classic fart effect for a second gag, keeping this clip first so the sequence builds.
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Honkie Ponkie is a very short sound effect clip lasting just over one second. Its name and quick runtime give a listener a silly, light feeling, more goofy than serious, and the joke lands as a brief cartoonish moment rather than something tense, relaxing or drawn out over a longer stretch.