Place it after the setup in a prank edit, then follow it with a shocked reaction before the laugh that follows closes the scene, keeping the reveal first and the payoff last.
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The J3VU clip runs for about fifteen seconds and is listed as a prank sound, made to be played at a chosen point rather than left running in the background. It gives a playful, off-balance feeling, as if something ordinary has suddenly gone sideways for the listener.