Place this clip at the reveal point of an edit, after the setup shot and before the reaction lands. Pair it with a bass drop sound effect for a heavier punch, or follow it with the fail sound meme when the villain plan immediately backfires.
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This clip is Jared Leto performing the Joker's laugh, a drawn-out, uneven cackle that lasts just under five seconds. It lands as tense and unsettling rather than friendly, with a smug, villainous edge that makes a normal moment feel threatening. It comes from his portrayal of the Joker in the DC films.