Place it right after the failed moment in your edit, then follow it with the Emotional Damage sound for a second beat before cutting to the Bruh sound as the closing beat. That order builds from one loss to a joke, then to a flat response.
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This is a short, two-second cry from Luigi, delivered as a high-pitched, wavering wail that sounds like he has just lost something important. It is funny because the distress feels so exaggerated, yet also a little pitiful, which makes it land as a joke rather than real sadness. The over-the-top emotion gives it a cartoonish, instantly recognisable charm.