Place it as the final beat after the failed attempt is fully shown, following a short Roblox Oof for the death itself, or follow a sudden FNAF Jumpscare Scream with it to turn a scare into a defeat.
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This six-second chiptune jingle marks the moment Mario loses his last life, opening with a few bright notes before falling into a low, resigned ending. It feels funny and final at once, turning a small slip into a dramatic defeat, and it carries a warm nostalgia for anyone who grew up playing the arcade original from Nintendo.