Place it right after the hit in a fail edit, then follow it with the Bruh Soundboard Button for the next beat, or cut to the Emotional Damage Sound Button when the loss keeps getting worse.
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This is a brief, just-over-a-second hurt sound linked to Sonic.exe, the distorted, horror version of Sonic, delivered as a sharp pained vocal reaction. It feels sudden, unsettling and darkly funny, like a cartoon hero wincing after a bad hit, with the shock landing before the pain has time to settle.