Place it at the very start of a clip as the interruption, then cut straight to the other person's reaction for the payoff. Follow it with Bruh Soundboard Button when the room goes quiet, or lead into Oh My God Bro Oh Hell Nah Sound Button if the interruption sparks a bigger argument.
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This short voice clip catches someone mangling 'excuse me' into a garbled 'sumimascuse me', delivered in one rushed, confused burst that lasts under two seconds. It feels awkward and funny, like interrupting at exactly the wrong moment with exactly the wrong words. The line comes from Two Best Friends Play Yakuza 0, part 42.