Place it at the exact moment a favour lands in your edit, after the helpful action and before your reaction shot. Follow it with an awkward moment beat if the thanks goes unanswered, or end the sequence there when the recipient reacts.
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A polite male voice delivers the line thank you kind sir in a calm, formal, slightly theatrical tone over about three seconds. It feels warm and sincerely grateful, with the exaggerated courtesy making listeners smile at how grand it sounds. The clip is associated with Oblivion and its famously formal NPC dialogue.