Place it as the immediate reaction beat after the line or mistake it is answering, before any commentary resumes. Follow it with a vine boom sound for a bigger reveal, or swap in a censor beep when the moment needs bleeping instead of disbelief.
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The clip is a short 1.5-second effect built around a drawn-out tch and hiss, like someone sucking their teeth in disbelief. It lands as dismissive and mildly exasperated, the kind of reaction that says you cannot believe what you just heard without needing any actual words to explain the frustration behind it.