Put this clip at the peak of a fail or reveal, after a tense build, then let the reaction shot play out. For a bigger alarm-style payoff, follow it with the Danger Alarm Meme Sound, or swap in the Inception Horn Sound Effect when you want a heavier hit instead.
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This is a very short air horn blast, just over a second long, with a harsh brassy blare that hits instantly and cuts through everything around it. It feels loud, silly and deliberately over the top, like a fake alarm going off at the worst possible moment. The sudden jolt makes people laugh, flinch or look up at once.