Place it at the turn into the next room, after a Dun Dun Dun build and before a Vine Boom impact, so the edit moves from build-up to payoff in one clean run.
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This is a short, roughly two-second clip carrying the A-183 sound from Interminable Rooms. It gives a tense, unsettling jolt that fits a chase, a close call or a sudden reveal, making a quiet scene feel dangerous in an instant.