Place it at the turning point of a reveal edit, right after the plain setup has been shown. Put It Was At This Moment He Knew just before it to build the realisation, then follow it with clapping once the identity is out in the open.
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This four-second cartoon voice clip has Dr. Doofenshmirtz spotting a plain platypus and then realising, in a sudden shout, that it is Perry. The quick switch from puzzled to alarmed makes it feel funny and surprising, like a secret identity has just been exposed in front of everyone. It comes from Phineas and Ferb, where the line recurs whenever Perry appears in disguise.