Place it at the peak of the reaction, right after the visual punch lands, then follow with a scream meme variant for a second hit or an emotional damage sting to close the joke. Keep it as the top layer so the paired buttons answer it instead of competing underneath.
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This clip is a 2.3-second stretched-out human scream, just a long aaaaaaaaaah held with frantic energy and a row of exclamation in its original title. It feels panicked, funny and completely over the top, the sound of a reaction blowing up way bigger than the moment that caused it.