Place this clip right on the reaction cut itself, after your opening gag and before the reply line lands. Follow it with the Mayuri Tuturu Sound for a second vocal beat, or swap in the Anime Ahh Sound Effect when you want a matching reaction from another character.
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This is a one-second clip of an anime-style female voice giving a soft, high-pitched "ah." The delivery is bright and exaggerated, and it feels playful and endearing, turning a small visual beat into a bashful, startled, curious or adorably confused reaction without overwhelming the scene around it.