Place it straight after the mishap, as the first reaction in the edit, then follow with Ba Dum Tss for the punchline. For a longer roast, open with Oh Brother This Guy Stinks and save this clip for the smaller second hit that follows the main joke.
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This is a very short vocal clip, lasting about 1.3 seconds, built around one stretched, high-pitched 'aii'. It sounds like a sudden yelp, half surprise and half playful complaint, the kind of noise someone makes when they touch something unexpectedly hot. It feels funny, light and a little dramatic, landing as a quick wince rather than a serious cry.