Place it at the end of a death reveal, after a Dun Dun Dun sets up the moment and before an Anime Wow captures the audience reaction. That order keeps the focus on Annie, with the other two sounds framing the scene instead of competing with it.
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This 2.6-second clip is built around Annie's death, a single dramatic moment presented on its own. It feels tense and sombre, turning a character loss into something that lands with real weight. Played at the right moment, it gives a scene a sharp emotional punch without needing any explanation.