Use it as the middle beat in a three-part gag, between a slow build and a hard impact sound. Pair a slow Dun Dun Dun with it first to set up the scare, then follow the scream with a Vine Boom to land the punchline and close the moment.
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This is a short human scream, about four seconds long, that climbs steadily in pitch and intensity instead of staying flat. The rising shape makes it feel panicked and out of control, like a reaction that keeps getting worse. It works as a burst of alarm, shock or over-the-top distress, with the climb doing most of the comic and dramatic work.