Place it just before the reveal in your edit, then follow it with Dun Dun Dun for the twist and Vine Boom Sound for the payoff, so the sequence builds, turns and lands in that order.
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This is a suspense sound effect made to hold a moment in tension, with a dark, uneasy tone that keeps listeners waiting for what comes next. It feels tense, watchful and slightly ominous, as if a reveal is approaching and nobody in the room wants to look away. It suits a dramatic pause, a plot twist or a sudden turn in a story.