Place it at the end of a failed moment, after the action has finished and before any reaction shot. Pair it with the Classic Error Sound for a double miss, or follow it with Dun Dun Dun when the failure needs a bigger payoff, then cut straight to the next scene.
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This is the Basso alert from Mac OS X, a single low-pitched bass note lasting about a third of a second. It lands bluntly and dryly, with a flat finality that feels understated rather than dramatic, like a quiet tap on the shoulder instead of a shout. As one of the classic Mac system sounds, it is instantly familiar to anyone who has used a Mac.