Place it at the end of a clip, just as the heavy load or failed chore is shown, then follow it with a Vine Boom to underline the collapse. If the joke is about a clumsy attempt instead, pair it with a SpongeBob Fail after the bray for a second beat.
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This is a short, roughly two-second donkey sound built around a pack donkey's bray. It feels comic and a little exasperated, like an animal refusing to move or complaining about the work in front of it. The brief burst makes it easy to recognise at once and hard to mistake for a calmer farmyard noise.