Place it at the end of a joke or fail, after the setup has landed, then follow it with the Laughing Dog Meme Sound Button for a second wave of laughter, or cut to the Gato Riendo Sound Button when the joke needs a different laugh. Keep it as the main reaction and let the second laugh answer it.
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This clip is Caio laughing out loud for about seven and a half seconds, his chuckle building into a loud, breathless burst that keeps going until he can barely catch his breath. It feels warm, silly and completely unguarded, the kind of laugh that makes a room laugh along with him. It works as a pure reaction, honest and infectious rather than performed.