Place it at the end of a setup line so the challenged remark lands first, then follow it with the Vine Boom sound for a heavy punch. For a failed comeback, add the SpongeBob Fail sound straight after it instead, keeping this clip as the setup and the second sound as the payoff.
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This is a short voice clip of CaseOh asking 'What did you just say?' in a sharp, incredulous tone, lasting just under three seconds. It lands with stunned disbelief mixed with playful confrontation, like catching someone mid-insult and making them repeat it. The delivery makes an awkward or outrageous remark feel even bigger, turning a quick challenge into the whole joke.