Place it right after the teasing peaks in your edit, then follow it with No No Wait Wait for a second protest, or cut to Oh My God Wow when the joke finally lands.
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This is a very short voice clip, lasting about 1.7 seconds, in which a young voice pleads 'stahp' in a drawn-out, whiny tone. It sounds like a half-laughing, half-annoyed protest from someone who has had enough, and that exaggerated pleading makes it feel funny, awkward and instantly relatable whenever somebody keeps teasing, poking or pushing a joke too far.