Put it just before the reveal in your edit, then follow the payoff with a Dun Dun Dun hit and a Vine Boom when the joke lands, keeping the reveal first and the punch second.
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This is a short dramatic sound effect lasting about five and a half seconds, designed to heighten a reveal or a turning point. It creates a tense, suspenseful feeling, as if something important is about to happen, and it suits moments that need weight and anticipation without a spoken line to carry them.