Place it at the end of a death scene, then follow it with Re:Zero Return By Death for a respawn gag, or cut to JoJo To Be Continued when the defeat deserves a cliffhanger instead of a restart.
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This 2.5-second clip from Sword Art Online Abridged captures a character's drawn-out dying cry, starting with a strained gasp and rising into a long, wailing 'Ovaaaahh'. It feels sudden, darkly comic and over-the-top, like a dramatic game-over moment played for laughs rather than real sadness. It comes from the fan-made abridged parody of Sword Art Online.