Place it as the payoff after the mistake is shown, following a short warning sound and before the comedown. Open with Knuckles Oh No to set up the trouble, then let this clip carry the reaction, and close with Sad Violin to finish the scene.
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This clip is a single drawn-out shout of 'noooo' from Delfin, lasting about five and a half seconds. It feels dramatic and exasperated, like a cartoonish refusal when something has just gone badly wrong, and its over-the-top length makes the disappointment funny rather than serious.