Put it just after the punch sound that starts the hit, then let the bone crack follow it so the defeat builds in two steps before a SpongeBob fail closes the sequence.
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This clip is a brief male scream, lasting about three and a half seconds, delivered with raw, forceful energy. It gives a listener an immediate jolt of comic panic, as if a small mishap has suddenly become a disaster. It is commonly identified online as a Mortal Kombat male scream, which explains why it fits exaggerated defeats so well.