Place it right after the failure lands in an edit, then follow it with Scream Meme for a second reaction or Emotional Damage when the joke needs a follow-up payoff. Keep it as the punchline, not the opener, and let the next clip carry the aftermath.
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This short clip is a single drawn-out human scream, lasting about two seconds, with the voice rising and holding on one long, exasperated ahhhhhhh. It sounds raw and unfiltered, like someone giving up in the middle of a bad moment. The effect is funny and dramatic at once, turning frustration into an instant, over-the-top reaction that lands because it feels so familiar.