Place this clip as the immediate reply after the formal greeting in your edit, then follow it with the dun dun dun sound to underline the fallout, or precede it with the vine boom sound for a heavier reaction cut.
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This is a short 1.7-second voice clip of a speaker asking, 'Did you just call me sir?' in an offended, incredulous tone. It feels funny and awkward because the polite title suddenly lands as an insult, making the whole exchange feel instantly confrontational. The clip was taken from audio titled Get Off The Couch.