Place it at the very start of a night sequence, before any dialogue begins, then follow with low scene audio only. For a reveal, put this first and bring in a dun dun dun sound right after the cut so the second sound carries the payoff.
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This clip is a single distant wolf howling, lasting just under six seconds, with the call sounding far away rather than close and aggressive. It gives a listener a quiet, eerie and slightly lonely feeling, the kind of cold night-time mood that makes a scene feel empty, wild and a little unsettled without being frightening.