Put this clip at the end of a fail sequence, after the mistake is visible and before the reaction shots. Follow it with the Law and Order Dun Dun for a mock-serious verdict, or lead into it with the Windows XP Error Music when a computer plan goes wrong.
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This three-second Family Guy clip pairs a worried 'oh no' with a resigned 'I know', delivered in the show's familiar animated voices. It feels sheepish and knowing, like admitting a mistake you saw coming. It comes from Family Guy, the long-running animated television series.