Use it as the final beat of a clip, after the action has finished and before any closing caption appears. Put Dun Dun Dun just before it as a lead-in, then let Vine Boom land after the last word to close the clip.
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This is a short spoken farewell from Thragg, lasting about 3.3 seconds, delivered as a single cold goodbye with no extra dialogue around it. It feels final and menacing, like a door closing for good on someone who will not return.