Place it at the end of a reveal or betrayal scene, after the plan is exposed, so it closes the moment. Pair it with Enemy Spotted just before the reveal to build suspicion, then follow the laugh with FNAF Jumpscare Scream for a sudden shock payoff.
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This is a short, sharp laugh lasting about three and a half seconds, delivered in a mocking, over-the-top villain style that feels deliberately theatrical. It lands as funny and unsettling at once, like someone revelling in a plan going exactly as intended, and it works best when the joke needs a burst of pure, gleeful menace.