Put this clip right after a sudden suspense build in a scare edit, then follow it with a wrong answer gameshow sting for fails, or a cartoon slip effect when the mistake turns physical and clumsy on screen.
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This clip is a single under-half-a-second 'gaah' scream, a high-pitched human voice that jumps in with no words around it. It feels startled and panicky but also funny, like shock you can hear before you can think. It comes from GiantWaffle's live streams on Twitch, where it was captured as a gameplay reaction.