This clip is mainly used online as a reaction sound for defeat, failure and abrupt endings. The repeated “game over” wording makes it easy to fit into gaming edits, but it also appears in general meme videos far beyond game content. Its popularity comes from how clearly it signals that someone has lost, while the upbeat ending keeps it funny instead of harsh. It often fills the same role as GTA SA Mission Passed or Victory, except flipped into a losing joke rather than a win screen.