Place it just after the boast or reveal, then follow the fall with the Womp Womp Fail sound for the loser and close the scene with the JoJo To Be Continued sound to leave the rivalry hanging.
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This is a short 2.3-second burst of haughty, theatrical laughter from Gilgamesh, a proud king who laughs as if every rival is beneath him. It feels smug and triumphant, like a villain savouring someone else's failure, and it comes from anime, where his overconfident laugh has become instantly recognisable.