Place it just after a villain reveal or a failed scheme, then follow it with a Vine Boom to underline the moment, or open with Dun Dun Dun before the laugh to set up the scene. For a bigger flop, pair it with SpongeBob Fail right after the laugh lands.
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This is a short, roughly 1.7-second burst of several henchmen laughing together in a loud, mocking chorus, with overlapping voices that rise and fall as one. It feels sly and villainous, like a room full of bad guys enjoying someone else's bad luck, and it lands best as a quick, knowing chuckle rather than a warm laugh.