Place it at the very end of a fail sequence as the closing reaction. Follow a Windows XP Error Sound with this clip, then let a SpongeBob Sad Song carry the aftermath if the scene needs to linger a beat longer before you cut away.
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This clip is Homer Simpson letting out a long, drawn-out moan with no words, lasting just under five seconds. It feels weary and theatrically fed up, turning embarrassment, annoyance and instant regret into something funny rather than heavy. It comes from Homer in the animated series The Simpsons, whose exaggerated vocal reactions are instantly recognisable.