Place it at the end of a short reaction sequence, after the offending line has finished, so it acts as the final verdict. Pair it with the SpongeBob Fail sound first for a failed plan, then follow with the Vine Boom sound when the moment needs a heavier punch.
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This short clip captures a grumbling vocalisation that sounds like someone saying 'ieh bieh' in exasperation, lasting just under four seconds. The irritated delivery makes it feel petty, tired and deliberately unimpressed, as if the speaker has heard the same nonsense one time too many. It works as a quick, wordless way to show annoyance without starting an argument.