Place it at the end of a setup, right after the other person has finished their long explanation, so it acts as the cut-off. Follow it with the Sad Violin sound for a mock-sympathetic exit, or put the Wrong Answer Game Show sound just before it when a bad take triggers the dismissal.
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This short voice clip is the line 'I've lost interest', spoken in a weary tone and lasting just under four seconds. It gives a blunt, unimpressed feeling, a flat dismissal that makes a rambling story or an overlong pitch feel suddenly pointless, because the speaker sounds genuinely done listening.