Place this clip at the peak of a reaction sequence, right after the reveal and before the cutaway. Follow it with the JoJo To Be Continued Sound Button to freeze the scene, or precede it with the Windows XP Error Sound Button for a failure build-up.
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The clip is Joseph Joestar's dramatic English 'OH MY GOD' shout, a short voice line delivered at full volume in shocked disbelief. It feels hilariously over the top, turning a small surprise into a huge reaction. It comes from JoJo's Bizarre Adventure, where his exaggerated exclamations became a signature.