Place this clip as the immediate answer to the offending moment, then follow it with the Brother Eww reaction sound to extend the disgust beat, or lead into it after a Damn meme sound when the reveal needs a two-step rejection before the scene moves on.
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This is a sub-second effect built from a sharp throaty ha followed by a wet tfu, with no speech, music or background noise behind it. The gesture feels rude, dismissive and deliberately exaggerated, so listeners read it as comic contempt and disgust rather than a realistic spit, landing instantly as a wordless put-down.