Place it as the final beat in a short edit, after a short setup using Vine Boom Sound and before the reaction shot. Follow it with MLG Air Horn only if the joke needs a second payoff, and keep Vine Boom Sound earlier so the ending stays the clear payoff in the sequence.
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This is a single, extremely loud sound effect that runs for just over seven seconds, built around one huge, overwhelming burst of noise with no voice or music in it. It feels startling and almost physical, the kind of blast that makes people flinch, laugh, or turn around at once. It works best as a shock moment, not as background, because its whole point is the sudden impact.