Place it at the peak of a fail, after the setup and before the reaction, then follow it with Bruh for a deadpan beat or Emotional Damage when the loss keeps getting worse, and save Scream Meme for a bigger scare later in the same edit.
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This is a short, roughly three-and-a-half-second clip of Luigi letting out a scream pitched down low and deep, stretching his familiar panicked yell into something heavier and more dramatic. It feels funny and overblown, like a small scare turned into a full-blown meltdown, and it works because the familiar voice sounds suddenly wrong in the best way.