Place it as the last beat of a scene, after the failed plan has been shown in full. Open with You What SpongeBob for the confused reaction, then follow with this refusal, and close with Law and Order Dun Dun to end the scene.
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This is a short spoken Italian line, lasting about two and a half seconds, delivered with a firm, exasperated tone. It sounds like a final refusal, the kind of emphatic never again that closes an argument or a bad idea for good. The bluntness makes it funny and satisfying, especially when the listener already knows the speaker means it.